FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10278302
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC274830.2673885239020998No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC232020.2257376753475428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227460.22130114487782124No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA225080.21898558730809817No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA217110.2112313882195717No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG135440.13177273833751918No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA121350.11806424835541901No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG114200.11110784641276351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAGC33850.039.1264885
GCGTTCT33850.038.8638610
GAGCGTT34750.038.3008358
CGTTCTC34700.038.2286711
GACACGT35350.038.08878721
ATACCGC12700.037.93717635
CGGAGCG35250.037.6351246
AGACACG36550.037.0186720
CGGCCTA37350.036.87119310
TATACCG13200.036.49980534
ATGCGCC33500.035.98804531
ACGTGCC36900.035.77390724
CTACTCG73800.035.1470072
ACACGTG38500.034.6868822
GTCGAAT16150.034.59908710
TACTCGG76950.034.3658263
GTCCTAC38250.034.33757419
TTCGGAG38850.034.317424
TCCGACG11550.034.09505541
CGGAACT16850.033.947232