FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11143121
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGAACGGTAATCTCGTATGCC294000.26383990625247633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277540.2490684611609261No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA218560.19613894527394973No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA174620.15670654567961703No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGAACGGTAATCTCGTATGC173800.1559706656689809No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAACGGTACTCTCGTATGCC132050.11850360415183502No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA131050.11760618950471775No Hit
CCCACTGTGGTGCCTGGTGGAGACCTGGCCAAGGTACAGAGAGCTGTGTG119350.10710643813344574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC70650.035.0946844
GCACTTT271600.034.2637021
GAATCGC100800.033.6351325
AGAATCG104050.033.59904524
AGGCTGA590400.033.5721211
CTACTTG140400.033.4546432
TTGGGAG471800.033.2989586
TACTCGG126350.033.1701433
AATCGCT103750.033.1647126
AGGCCGA129700.032.955911
GCTACTC266800.032.909371
TCCGACG10250.032.83880641
CTACTCG128250.032.815822
GCTACTT146250.032.6879431
TTTGGGA330550.032.5659455
TTGAACC266750.032.40361832
GGAGGCT626300.032.0553369
GAGAATC206350.031.9858623
TACTTGG150150.031.9417533
GAGGCCG135550.031.69589610