FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8477857
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213460.251785327353363No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA190990.22528098787228898No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA138740.1636498468893731No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA96670.11402645739365502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGCAACGATCTCGTATGCC89010.10499115519405436No Hit
GACAGAAATCAGGTATTGGCAGTTTTTCCATTTTCATTTGTGTGTGAATT88520.10441317894368823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG6800.035.5884541
GAATCGC91800.035.0624625
AGAATCG95450.035.0350924
GCACTTT224550.034.869891
TACTCGG114300.034.8582273
GCTACTC237100.034.6665761
CTACTCG116000.034.4801332
AATCGCT94600.034.42003326
AGGCCGA102800.034.30383711
AGGCTGA524350.033.93718711
CTACTTG129850.033.9199452
GAGCGTT13550.033.7698338
TTGGGAG408100.033.683146
TCGGAGC13200.033.3343285
GCTACTT135450.033.2811661
TTTGGGA273400.033.2745825
GAGAATC184250.033.1828823
CGGAGCG13400.033.001186
CGCTTGA108050.032.90484629
GCGTTCT13850.032.87951710
Produced by FastQC (version 0.11.5)