Basic Statistics
Measure | Value |
---|---|
Filename | HFJM7BGXC_n01_S_20.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11276232 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGCC | 33469 | 0.2968101401248219 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 26930 | 0.23882091109867198 | No Hit |
TCTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGC | 19210 | 0.17035832536968024 | No Hit |
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA | 17353 | 0.15389005831025823 | No Hit |
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC | 13579 | 0.12042143155621488 | No Hit |
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA | 12301 | 0.10908785842646729 | No Hit |
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG | 12194 | 0.10813895989369499 | No Hit |
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC | 11619 | 0.10303973880636723 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCCGACG | 1860 | 0.0 | 36.783875 | 41 |
GAGCGTT | 1970 | 0.0 | 35.39904 | 8 |
GTATGCC | 6785 | 0.0 | 35.049515 | 44 |
TCGGAGC | 1995 | 0.0 | 33.745327 | 5 |
CTACTCG | 6890 | 0.0 | 33.40008 | 2 |
TTCCGAC | 1990 | 0.0 | 33.276447 | 40 |
GCGTTCT | 2040 | 0.0 | 33.21354 | 10 |
CGACGGC | 2075 | 0.0 | 33.184578 | 43 |
TACTTCG | 2165 | 0.0 | 33.02503 | 17 |
TACTCGG | 6990 | 0.0 | 32.859306 | 3 |
AGGCTGA | 33900 | 0.0 | 32.34894 | 11 |
GAATCGC | 5925 | 0.0 | 32.194324 | 25 |
GCGACGT | 890 | 0.0 | 32.133183 | 13 |
CGGAGCG | 2100 | 0.0 | 32.058056 | 6 |
AGAATCG | 6015 | 0.0 | 32.005234 | 24 |
GCTACTC | 14900 | 0.0 | 31.908293 | 1 |
AATCGCT | 5965 | 0.0 | 31.6828 | 26 |
GCACTTT | 16525 | 0.0 | 31.57972 | 1 |
TGCGCGA | 900 | 0.0 | 31.534235 | 4 |
CGGATCA | 3995 | 0.0 | 31.336033 | 24 |