FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11276232
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGCC334690.2968101401248219No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269300.23882091109867198No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGC192100.17035832536968024No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA173530.15389005831025823No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC135790.12042143155621488No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA123010.10908785842646729No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG121940.10813895989369499No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC116190.10303973880636723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG18600.036.78387541
GAGCGTT19700.035.399048
GTATGCC67850.035.04951544
TCGGAGC19950.033.7453275
CTACTCG68900.033.400082
TTCCGAC19900.033.27644740
GCGTTCT20400.033.2135410
CGACGGC20750.033.18457843
TACTTCG21650.033.0250317
TACTCGG69900.032.8593063
AGGCTGA339000.032.3489411
GAATCGC59250.032.19432425
GCGACGT8900.032.13318313
CGGAGCG21000.032.0580566
AGAATCG60150.032.00523424
GCTACTC149000.031.9082931
AATCGCT59650.031.682826
GCACTTT165250.031.579721
TGCGCGA9000.031.5342354
CGGATCA39950.031.33603324