FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7861728
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC241520.307209814432654No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC229060.29136088147542116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185930.23650016891960646No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA182480.23211182070913672No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA182150.23169206566291786No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG126910.16142761489586005No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA110840.14098681612999076No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG92630.11782396948864168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCT32250.040.5185610
CGTTCTC33200.039.95549411
CGGAGCG33650.039.8810776
TCGGAGC34100.039.2257545
GAGCGTT34400.039.009598
CGGCCTA28650.037.93146510
GACACGT27050.037.81976721
GTCCTAC35750.037.29273619
ATACCGC10250.037.13213335
TATACCG10300.036.95187834
AGACACG28050.036.70676820
TTCGGAG37350.036.165954
ACGTGCC28400.035.94544224
ACACGTG28550.035.60201322
ATGCGCC26450.035.4326231
CACGTGC29050.035.1407123
CCTTCGG38650.035.0633432
GGAGCGT38800.034.982717
GTCGAAT12400.034.7721610
TCCGACG12950.034.65654441