Basic Statistics
Measure | Value |
---|---|
Filename | HFJM7BGXC_n01_S_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7861728 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC | 24152 | 0.307209814432654 | No Hit |
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC | 22906 | 0.29136088147542116 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 18593 | 0.23650016891960646 | No Hit |
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA | 18248 | 0.23211182070913672 | No Hit |
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA | 18215 | 0.23169206566291786 | No Hit |
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG | 12691 | 0.16142761489586005 | No Hit |
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA | 11084 | 0.14098681612999076 | No Hit |
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG | 9263 | 0.11782396948864168 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGTTCT | 3225 | 0.0 | 40.51856 | 10 |
CGTTCTC | 3320 | 0.0 | 39.955494 | 11 |
CGGAGCG | 3365 | 0.0 | 39.881077 | 6 |
TCGGAGC | 3410 | 0.0 | 39.225754 | 5 |
GAGCGTT | 3440 | 0.0 | 39.00959 | 8 |
CGGCCTA | 2865 | 0.0 | 37.931465 | 10 |
GACACGT | 2705 | 0.0 | 37.819767 | 21 |
GTCCTAC | 3575 | 0.0 | 37.292736 | 19 |
ATACCGC | 1025 | 0.0 | 37.132133 | 35 |
TATACCG | 1030 | 0.0 | 36.951878 | 34 |
AGACACG | 2805 | 0.0 | 36.706768 | 20 |
TTCGGAG | 3735 | 0.0 | 36.16595 | 4 |
ACGTGCC | 2840 | 0.0 | 35.945442 | 24 |
ACACGTG | 2855 | 0.0 | 35.602013 | 22 |
ATGCGCC | 2645 | 0.0 | 35.43262 | 31 |
CACGTGC | 2905 | 0.0 | 35.14071 | 23 |
CCTTCGG | 3865 | 0.0 | 35.063343 | 2 |
GGAGCGT | 3880 | 0.0 | 34.98271 | 7 |
GTCGAAT | 1240 | 0.0 | 34.77216 | 10 |
TCCGACG | 1295 | 0.0 | 34.656544 | 41 |