FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8524946
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC235240.27594309688295976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199610.23414811073290084No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA175440.20579602498361865No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGC127670.14976047942121862No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG114380.13417093785696707No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC112130.13153162495105541No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC108380.1271327701078693No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC106610.12505651062188547No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA96780.11352564579294695No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC89120.10454025163326548No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA89070.10448160023535634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG14850.036.29453741
GAGCGTT17800.035.470198
GTATGCC60700.035.3726744
GACACGT15850.034.42366821
GCGTTCT17650.034.15091710
GAATCGC37400.033.94430525
TCGGAGC17700.033.5612375
CGACGGC15950.033.37808243
TGCGCGA8100.033.1376954
TACTTCG16750.033.09670617
CTACTCG42200.033.0538252
TACTCGG43350.033.0397533
AGAATCG39500.032.91949524
CGGAGCG17950.032.7264826
ATTCGAC5400.032.59082416
GCGACGT8400.032.47423613
TTCCGAC15850.032.34007340
CGTGATA20350.032.325622
AGACACG17050.032.25801520
AATCGCT39750.032.2133526