FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11834691
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG343430.29018924110481636No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC239220.20213455509738276No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA176320.14898572341263494No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA150400.12708401089643998No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC124900.1055371872404611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG11750.036.8844941
CTACTCG89550.036.386152
GAGCGTT18600.035.7198338
TACTCGG91900.035.503593
GCTACTC189150.034.650311
GAATCGC70600.034.18558525
CGACGGC12550.034.18240743
CGGAGCG19850.033.805966
AGAATCG71950.033.78864724
AATCGCT71500.033.63191226
AGGCTGA404100.033.6000311
CTACTTG102850.033.413612
GCGTTCT20250.033.35196310
ATACCGC10350.032.94714435
GCTACTT107450.032.9452321
TCGGAGC20450.032.9215475
TCGCTTG82550.032.6481328
GCGACGT6850.032.4361813
GCCTACG10700.032.074238
TATACCG10650.032.01905434
Produced by FastQC (version 0.11.5)