FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12884149
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGCC617040.478914051676987No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC414280.3215423851431709No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA411200.31915185085177145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG353060.2740266353641207No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCACTCTCGTATGCC300140.23295290981189365No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGC277570.2154352607999178No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG167880.13029964183121445No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT160160.12430778315277166No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGTCTACCACTCTCGTATGC152540.11839353922404965No Hit
CAATAAAGCTTGCCTTGAGTGCTTCAAAAAAAAAAAAAAAAAAAAAAAAA142640.11070967900169426No Hit
GATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACA136740.10613040876816932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG56300.038.9192962
GTATGCC144150.038.61149244
TACTCGG57450.037.565983
GCTACTC119650.036.9939771
GCCTACG21100.036.0739948
TCCGACG4700.035.57376541
GCGGTTA7150.035.07840329
CCTACGT21550.035.014469
TCACGGG20350.034.594043
TCGCCGA7200.034.2220766
CTCACGG21100.034.1983762
CGCATAC4650.034.0655635
AGGCTGA255500.033.69104411
CACGGGC20800.033.634344
ACGGGCA21050.033.130375
CTCGGGA68350.032.991685
ACTCGGG65800.032.8994264
CCGTAAC14650.032.8867241
TCGGGAG72400.032.817546
CGTAACC14350.032.80745742