FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9847128
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC334260.3394492282419808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281860.28623574305117183No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA193110.1961079413205556No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA161170.16367208794279914No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG143310.14553481989875627No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGCC131490.13353131999502799No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC121930.12382290552128498No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA115210.1169985807029217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG17500.037.4642376
CTACTCG69300.035.9381942
TCGGAGC18200.035.9026035
CGTTCTC18850.035.82846511
CGCATAC2000.035.2009435
GAGCGTT18750.035.1984378
GCGTTCT18750.035.19825710
GCTACTC143250.035.0943221
TACTCGG72150.035.0064963
GCGACGT4600.034.43307513
CTACTTG77700.034.2332652
CGGCCTA19800.034.10942510
AGGCTGA297100.033.89061411
GAATCGC52150.033.6656725
GCTACTT82250.033.6236341
AGACACG19450.033.5935320
AGAATCG54950.033.2711324
ACGTGCC19400.033.2276924
CGTACTA5900.033.1854742
GACACGT19700.033.16772521