FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10030662
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC355310.35422387874299827No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA282290.28142708826197116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263840.2630334867230099No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA210430.20978675186144247No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA178030.1774857930613154No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC177890.1773462210171173No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG153920.15344949316406037No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG137730.13730898319572526No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC119170.11880571790775125No Hit
GCAAATGCCAGGTCTAGCAAACATAATGCTAGTCCTAGATTACTTATTGA111920.1115778799046364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG26600.038.9560286
TCGGAGC27700.037.567515
AGACACG27000.037.4003520
GCGTTCT27550.037.37034610
GAGCGTT28150.037.35558
CGTTCTC27800.037.35111
CGGAACT10200.036.6673742
GACACGT27300.036.58660521
ACGTGCC27550.036.09524524
CGGCCTA29500.034.90010510
CTACTCG45750.034.623622
TACCGAT4650.034.53898625
TCCGACG9100.034.32964741
ATGCGCC26150.034.24140531
GCTACTC99350.033.4822771
TTCGGAG31150.033.477374
GGTCGCT24500.033.402737
ACACGTG30100.033.40213422
TACTCGG47450.033.3367843
TAAGCGC5900.033.1855917