FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10244819
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG256660.25052663204689124No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC200100.19531823841885346No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC186860.18239463283831564No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA182420.17806073489438906No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC154280.15059319252004355No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA141750.13836262016927775No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA122200.11927980377203345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCT22300.037.9806210
CGGAGCG23100.036.858446
TCGGAGC23450.036.3079645
GAGCGTT23050.036.0768978
GACACGT25350.035.58241321
CTACTCG41000.035.4690252
AGACACG26150.035.1667320
TCCGACG11050.034.8418441
CGTTCTC24400.034.71162811
TACTCGG42500.034.3724753
GTCGAAT12900.033.76616310
CGGCCTA28150.033.7607110
ATGCGCC25100.033.39380631
AATCGCT33650.033.01767326
ACGTGCC27550.032.8221324
TCGCCGA6900.032.5228846
GCTACTC94100.032.4515721
GAATCGC33600.032.2816925
CGACGGC11800.032.06830243
ATACCGC11400.032.03465735