FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10295966
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286420.27818662182839377No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC235930.2291479983519759No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA210080.20404107783572714No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC172070.16712370650796635No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC169580.16470528360330638No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA159760.15516756756966757No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA132210.12840951494983568No Hit
ACTGTGGAGGAAGGATTGTAAAAAAAAATGCCTTTGAGACAGTTTCTTAG109710.10655629593182417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG25250.038.5114676
GCGTTCT25900.037.28759410
GAGCGTT26150.037.0156028
TCGGAGC26350.036.9872675
CGTTCTC26450.036.3457211
GACACGT25600.035.92293521
AGACACG26400.035.2508720
CTACTCG36450.035.0673982
TCCGACG11000.034.7998441
CGGCCTA27800.034.0269710
CGGAACT12850.033.5565952
ACACGTG27650.033.33865422
ACGTGCC27700.033.2797724
TCCGAAG12400.033.178421
TACTCGG38550.033.157273
CGTACTA4800.032.9995342
TTCGGAG29850.032.9452134
GCTACTC78950.032.4639131
GTCGAAT13050.032.36609610
TGCCGTA8900.032.13515539