FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10603993
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237940.22438717188892904No Hit
GACAGAAATCAGGTATTGGCAGTTTTTCCATTTTCATTTGTGTGTGAATT167610.1580630994381079No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC155450.14659572106469704No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA142710.134581378920186No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC127710.12043576415035355No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAGCCTCATCTCGTATGCC126490.11928525414907384No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA116720.11007174372898963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG15400.037.28550341
CGGAGCG20650.036.0111776
GAGCGTT21650.035.3613858
CGACGGC16000.035.19996643
GTCGAAT9500.035.1984710
TCGGAGC21850.034.3351975
CTACTCG29050.034.231412
TTCCGAC16500.033.46726640
GCGTTCT22500.033.4385510
TACTTCG18200.032.8781617
CGGAACT10200.032.7850572
TACTCGG30250.032.728173
GAATCGC24600.031.48142625
TGCCGTA17900.031.46469739
CGTTCTC24000.031.44000611
CCTTCGG24250.031.2089522
ATACCGC9600.031.16693135
TATACCG9800.030.75536234
TTCGGAG24300.030.692344
AGGCTGA156550.030.15618511