FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139959
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG409070.3672096100174157No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCAACCTAATCTCGTATGCC317260.2847945849710937No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCAACCTAATCTCGTATGC165710.14875279163953833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG13550.035.7193541
GCTACTT230550.035.6028021
GTATGCC67250.035.5269344
CTACTTG226200.035.4606062
CTACTCG209700.035.3657152
TACTCGG210650.035.3315473
TACTTGG230100.035.0699233
GCTACTC423750.034.950911
CGACGGC13800.034.7534243
AGGCTGA880950.034.65567411
GAATCGC168000.034.5467425
AATCGCT170350.034.53449226
GCACTTT250800.034.517651
AGAATCG174250.034.46968524
TACTCAG210250.034.373323
TTGGGAG544650.033.8818366
GGAGGCT904850.033.424559
GAGAATC336250.033.24546423
CTCGGGA234500.033.2394875
CCGTAAC10550.033.15620441
Produced by FastQC (version 0.11.5)