FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7162386
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC202100.28216853992510316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179360.2504193434981024No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA152850.2134065379888769No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA142680.19920735911189372No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA139830.1952282381876654No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC137670.19221248338193447No Hit
GACAGAAATCAGGTATTGGCAGTTTTTCCATTTTCATTTGTGTGTGAATT136120.19004840007226642No Hit
GCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTCTAAAGTGTTGGT98880.13805455332901634No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG86640.12096527609654101No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA79160.11052182889891721No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA73590.10274509081191659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACG23250.038.5131920
GACACGT23300.038.14807521
ATACCGC8950.037.60869635
ACGTGCC24100.036.9743324
GTCGAAT10400.036.805410
GCGTTCT17950.036.7665110
CGGAGCG18250.036.6471066
TATACCG9100.036.50551234
CGGAACT10550.036.2848852
GAGCGTT18850.036.062178
ACACGTG24750.035.646722
CGAATGA10800.035.44223812
TCGGAGC19050.035.1081165
CGTTCTC19000.034.96624411
CGGCCTA27150.034.7602610
ACCTGCG26400.034.75055
ATGCGCC24150.034.70784831
CACGTGC25750.034.60437423
CCTTCGG20100.034.149752
TGCCGTA8900.034.11261439