FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9w-31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9w-31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13677515
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG647530.47342664219341013RNA PCR Primer, Index 26 (100% over 43bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT148640.10867471174405585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT103550.061.77239642
TCGTATG109000.060.93110343
TATGCCG110800.060.2506546
ACGTCTG150900.057.65749415
AGTCACA130950.056.50911728
CAGTCAC132750.056.34059527
ATGCCGT118250.056.19050647
GTATGCC120000.055.92016245
CGTATGC120750.055.25853344
ACACGTC159000.054.9594913
GTCACAT133000.054.85975329
CACGTCT161600.054.03254314
GTCTGAA162050.053.7535917
ACATGAG136450.052.46175832
CACATGA138500.052.0335931
CCAGTCA155950.050.12694526
CCGTCTT131250.049.99413350
GCCGTCT130300.049.85557649
CTCCAGT175250.049.27192324
TGAGCAT142900.049.1778835
Produced by FastQC (version 0.11.2)