FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9w-29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9w-29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20247246
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA786550.3884725853580284TruSeq Adapter, Index 25 (97% over 44bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT212060.10473523164582482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT101650.058.58134544
TCGTATG108800.057.04625345
TATGCCG111850.055.5194748
TATCTCG111200.053.7372541
GTCACAC119650.053.25439529
CGTATGC121150.051.34462746
ACGTCTG141800.050.60441215
CAGTCAC129800.050.09990327
AGTCACA128300.049.95963728
GTATGCC127250.049.07164447
ACTGATA129950.048.60922634
ATGCCGT129350.047.92812349
TCACACT134200.047.4034230
TATATCT128500.046.60883339
ACACGTC157350.045.82350513
ATATCTC132000.045.4786640
CACGTCT158650.045.40419814
GTCTGAA164550.043.71288317
CTGATAT147900.042.56998435
GCCGTCT148350.041.7430951
Produced by FastQC (version 0.11.2)