FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9w-28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9w-28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12136523
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG462630.38118825301117953RNA PCR Primer, Index 33 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT64150.058.24566742
TCGTATG72250.057.73248743
TATGCCG73100.057.0597746
GTATGCC79450.052.67364545
CGTATGC79450.052.5870144
ACGTCTG99150.050.69557615
ATGCCGT84350.049.89740447
CAGTCAC88000.049.19970327
CACGTCT105750.047.76034514
AGTCACC89950.047.5961728
ACACGTC111000.045.56413713
GCCGTCT92250.044.9887849
GTCTGAA112600.044.88494517
CCGTCTT93000.044.40339750
CCAGTCA101450.044.30918526
TCCAGTC115150.043.44156325
TCTCGTA87050.042.6460641
CTCCAGT120800.041.80955524
CACACGT121200.041.64430612
TCTGAAC124000.040.86970518
Produced by FastQC (version 0.11.2)