FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9w-27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9w-27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12408064
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG284860.22957650766469287RNA PCR Primer, Index 29 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50500.054.24487743
TATGCCG54100.050.7621746
CTCGTAT45100.050.56572742
ACGTCTG75150.049.12017415
CGTATGC60550.045.29800444
CAGTCAC66550.044.99611327
GTATGCC61650.044.3780345
CACGTCT84200.044.12779214
ACACGTC88800.041.91976513
ATGCCGT67150.040.99843247
GTCTGAA92400.040.28587717
CTAATCT57450.039.7545737
AGTCACC72300.039.69980628
CCAGTCA83100.038.56719626
CACACGT97150.038.1395312
TAATCTC60350.038.01574338
TCCAGTC95700.037.8873425
TCTGAAC103550.035.78141818
CCGTCTT77200.035.25886550
CTCCAGT104050.035.24475524
Produced by FastQC (version 0.11.2)