FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9w-26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9w-26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10554493
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG278180.26356547870181923RNA PCR Primer, Index 28 (100% over 43bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT106470.10087647033353474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42050.058.33676542
TCGTATG44400.057.34664543
TATGCCG46850.054.4921746
ACGTCTG60400.053.74676515
CAGTCAC57450.048.2795927
GTATGCC53250.048.00811845
ACACGTC68250.047.86841213
CACGTCT68750.047.41991814
GTCTGAA71100.045.9006817
ATGCCGT56500.045.73377647
AGTCACC62000.043.40107728
CGTATGC59950.042.6435544
CACACGT79100.041.0847112
CCAGTCA73750.039.76074226
GCCGTCT65150.039.50282349
CCGTCTT64700.039.4576550
TCCAGTC82100.039.4146125
CTCCAGT82800.039.37304724
TCTGAAC84700.038.69347818
CTGAACT88550.036.93323519
Produced by FastQC (version 0.11.2)