FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9w-24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9w-24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12559938
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA455210.36243013301498783TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57550.058.4502444
TCGTATG60650.057.90919545
TATGCCG63350.054.83982548
CAGTCAC71850.051.66383427
ACGTCTG85250.050.70622615
TATCTCG65750.050.690241
GTCACAT71350.050.6718429
GTATGCC69350.050.4931947
AGTCACA72700.050.44289428
ATGCCGT73100.047.6197449
CACGTCT91750.047.2647414
ACATTCC74700.047.24503332
TTATCTC73200.045.7196940
CGTATGC77350.045.5845546
ACACGTC95950.045.30387513
GTCTGAA96650.044.8320817
CCAGTCA91050.042.3985826
CACATTC85250.041.64092631
CCGTCTT83350.041.4320652
CACACGT106350.040.87359212
Produced by FastQC (version 0.11.2)