HFJJCBGX2_n01_9w-23.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HFJJCBGX2_n01_9w-23.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11006424
Sequences flagged as poor quality	0
Sequence length	75
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	129276	1.1745504261874702	RNA PCR Primer, Index 32 (100% over 43bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG	21187	0.19249667285214525	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	13532	0.12294638113160097	RNA PCR Primer, Index 32 (97% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	11790	0.10711926053366652	No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT	11570	0.10512042785195265	No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGT	11329	0.10293079750516607	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	17875	63.982594	43
CTCGTAT	15800	63.803936	42
TATGCCG	18075	63.255535	46
CGTATGC	18575	61.626568	44
ACTCAAT	18810	60.90973	35
GTATGCC	18850	60.764942	45
ATGCCGT	18965	60.3036	47
CAGTCAC	19825	58.991463	27
AGTCACC	19955	58.174946	28
GCCGTCT	19615	58.041435	49
CCGTCTT	19705	57.723816	50
ACGTCTG	21780	57.656216	15
CACTCAA	20200	57.145123	34
CCACTCA	20130	57.137928	33
CCAGTCA	20940	56.789394	26
TCTCGTA	17770	56.459015	41
CACGTCT	22450	55.90504	14
GTCTGAA	22530	55.82878	17
CACCACT	20805	55.549446	31
GCTTGAA	20795	55.26196	59