FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9w-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9w-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12214238
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG388610.31816147679454093RNA PCR Primer, Index 31 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG55150.057.6167843
CTCGTAT54600.056.4910642
TATGCCG60150.052.4249846
ACGTCTG74150.050.89896815
TATCTCG60500.050.8092739
ACGATAT63400.050.17127635
CACGATA65250.049.11830534
CGTATGC66550.047.74703644
CAGTCAC70150.047.112827
GTATGCC67200.047.0794545
CACGTCT82450.045.94267714
CGATATC67300.045.87979536
ACACGTC83900.044.9023313
AGTCACC72500.044.6815928
ATGCCGT70900.044.5709747
CCACGAT73450.043.58702533
GTCTGAA87650.043.25604617
ATATCTC72200.042.71863638
CCAGTCA81950.042.3075826
ACCACGA78000.041.13290832
Produced by FastQC (version 0.11.2)