FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11163821
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA506100.45333940771712483TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66600.058.01849444
TGAAACG72350.057.88809235
TCGTATG72750.057.7136245
GTCACGT74250.057.613829
TATGCCG73150.057.58513648
CACGTGA73800.057.49764331
TCACGTG76650.055.67481630
AGTCACG78500.055.37377528
ACGTCTG91050.055.20531515
GAAACGA77150.054.46536636
GTATGCC79550.053.08236747
CGTATGC80000.052.43996446
CAGTCAC84800.052.31766527
ACACGTC98500.051.2066113
CACGTCT98350.051.108414
ATGCCGT84750.049.6625749
GTCTGAA105350.047.6463617
CACACGT108250.046.49906512
AACGATC83050.045.98570638
CCAGTCA100950.045.28066626
Produced by FastQC (version 0.11.2)