FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10553661
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG488040.46243668429372514RNA PCR Primer, Index 17 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT65050.059.7735542
TCGTATG68750.059.06609343
AGTCACG71350.058.11763828
GTCACGT71500.057.2722429
TATGCCG70600.056.93055746
CGTATGC72500.056.00988844
CAGTCAC79300.053.29179827
ACGTCTG88850.053.19399615
CGTAGAG76600.053.008733
TCACGTA77000.053.00215530
CACGTAG78650.051.75863331
ACGTAGA78550.051.6927732
GTATGCC78900.051.11681445
CACGTCT94900.050.05727814
ATGCCGT80900.049.85145647
ACACGTC100850.047.1039713
CCAGTCA93900.046.8059726
TCTCGTA83250.046.78993241
GTCTGAA103950.045.533317
CCGTCTT89150.044.81227550
Produced by FastQC (version 0.11.2)