FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14188865
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG302570.21324468165705995RNA PCR Primer, Index 24 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG48400.053.03459543
AGTCACG49750.052.49311428
CTCGTAT45600.051.9784842
TATGCCG50850.050.13903846
CGTATGC56250.045.3263344
ACGTCTG72750.044.95483415
CACGTCT75500.043.5004914
GTATGCC60800.042.10439745
CAGTCAC67800.039.9937827
ACACGTC84150.039.19377513
GGTAGCA67600.037.9693734
TAGCATC62350.037.79193536
ATGCCGT68150.037.61245347
GTCACGG71000.036.5879229
CCAGTCA79650.036.25260526
GTCTGAA92700.035.57768617
CACACGT95250.034.77131312
CCGTCTT74150.034.47603650
TCCAGTC95550.034.2276625
CGTCTGA97750.033.66907516
Produced by FastQC (version 0.11.2)