FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12851019
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA397920.3096408152536386TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG67300.057.77091233
ACGTTTC70450.055.1388632
GTCACGT71950.054.99624329
TCGTATG68050.054.60238645
CTCGTAT59150.054.41925444
TATGCCG69000.053.4983248
TTTCGGA73000.053.16655335
CACGTTT74500.052.5581431
TTCGGAA73850.052.4611836
AGTCACG76750.052.3658228
TCGGAAT75800.050.97663537
ACGTCTG97650.049.14227315
CGTATGC76250.048.6395246
GTATGCC79750.046.97927547
CAGTCAC87400.046.81377427
CACGTCT106850.045.13703514
ACACGTC107200.045.08656313
ATGCCGT84750.043.31191649
GTCTGAA113550.042.47389617
CCAGTCA102900.041.9413426
Produced by FastQC (version 0.11.2)