FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12954560
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA803240.6200442160907048TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT102800.060.7442144
TCGTATG107950.060.4030145
TATGCCG108550.059.97147448
TCACGTG111750.058.9946230
AGTCACG113050.058.9570728
GTCACGT112850.058.75584429
GCCTTAT113000.057.09318537
ACGTCTG138600.056.85066215
TATCTCG109150.056.8011541
GTATGCC116550.056.09204547
CAGTCAC122850.054.8717327
CGTATGC119900.054.5263146
CCTTATC115050.053.61693638
ATGCCGT121950.053.43831649
CTTATCT115950.053.17182539
CACGTCT149350.052.89722414
ACACGTC149900.052.88766513
TTATCTC117700.052.58704840
GTCTGAA152300.052.00833517
CACGTGG128700.051.3857731
Produced by FastQC (version 0.11.2)