FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13140026
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA582540.4433324561153837TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT77550.059.79099744
TCGTATG82150.059.25674445
ACGTCCG82350.058.94278732
GTCACGT85700.057.20229329
AGTCACG86600.056.92650228
TATGCCG85500.056.44949348
CGCACAT86250.055.8796937
CGTATGC87250.055.79260346
ACGTCTG105400.055.708215
TCACGTC88000.055.51122330
CCGCACA87950.055.03368836
GTATGCC92100.052.62920447
CACGTCT113500.051.88551314
GTCCGCA94000.051.71124334
CAGTCAC98350.051.38820327
ACACGTC116750.050.58929413
ATGCCGT96600.049.9632549
GTCTGAA123150.048.01514417
TCCGCAC103150.047.1581435
TCTCGTA99650.046.53095643
Produced by FastQC (version 0.11.2)