FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10844451
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA491440.45317185720143877TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66300.057.1359944
TCGTATG69500.055.84556245
CACGAGT70950.055.33383631
TATGCCG69700.055.2372948
TATCTCG69900.054.096441
AGTCACG76100.052.49583428
CGTATGC74000.052.44906646
ACGTCTG88650.051.5247815
TCACGAG77750.050.98243330
ACGAGTG78100.050.31223732
GTCACGA80150.049.88624629
GTATGCC79750.048.44960847
ATGCCGT80200.048.0917349
CACGTCT95850.047.87055614
CAGTCAC84750.047.62632827
ACACGTC96500.047.4408613
GTCTGAA99300.046.44993217
GGATATC82050.046.2522738
GTGGATA84450.046.1216936
AGTGGAT86800.045.07173535
Produced by FastQC (version 0.11.2)