FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_9s-21or22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_9s-21or22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13417654
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA423080.31531592631618016TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC55950.056.66708438
TCGTATG63500.054.16726745
CTCGTAT59000.053.56226344
TATGCCG64400.053.2481748
TACGTAA65950.052.31153536
ACGTAAT66100.052.24521337
CCGTACG68050.050.89886533
CGTATGC70450.048.9697646
ACGTCTG86400.048.51341215
GTATGCC73700.046.76297847
CAGTCAC78750.045.95426627
TAATCTC70100.045.52437640
ACCGTAC77550.044.886132
ATGCCGT78400.043.91557749
CACGTCT96250.043.7640414
ACACGTC97800.043.1411513
GTCTGAA102450.041.01423317
CCAGTCA95200.039.49939326
AGTCACC91600.039.05582428
CACACGT109150.038.71846412
Produced by FastQC (version 0.11.2)