FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12045901
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG702080.5828372655561422TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG99950.060.094743
CTCGTAT96200.059.78341742
TATGCCG100900.059.1180946
AGTCACG106550.057.60029228
CGTATGC104250.057.284844
CACGGCT108550.055.6173631
GTATGCC108600.054.99001345
ACGTCTG127250.053.59852615
CAGTCAC116300.053.51296627
ATGCCGT111300.053.5001547
GCTACAT114200.052.23284535
GTCACGG122100.050.26462629
GTCTGAA139300.049.1601717
CACGTCT139700.049.0202214
TCTCGTA117350.048.86245741
ACACGTC140200.048.82099513
CCAGTCA133450.048.13525426
TCCAGTC141950.047.99938625
CCGTCTT124100.047.87060550
GCCGTCT124350.047.8300549
Produced by FastQC (version 0.11.2)