FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12016010
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG593570.4939826115324471TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT75800.061.0826242
TCGTATG84700.059.6337343
TATGCCG87050.057.744546
CGTATGC87500.057.7242544
AGTCACG91650.055.8600628
CAGTCAC95500.054.07772427
ACGTCTG104950.054.04072615
GTCACGA95600.053.33551829
GTATGCC95650.052.6975645
ATGCCGT96950.051.88202347
ACACGTC113200.050.28551513
CACGTCT113600.050.16898314
TCACGAT105250.048.37981430
CACGATC104800.048.25837331
GTCTGAA117900.048.0754917
CCGTCTT107650.046.5649150
GCCGTCT107700.046.51126549
CCAGTCA115250.046.18755726
CACACGT125450.045.320412
TCCAGTC125400.045.0626125
Produced by FastQC (version 0.11.2)