FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10172425
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG261160.25673327648028865TruSeq Adapter, Index 16 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG43250.054.8018543
CTCGTAT40700.053.2343542
TATGCCG44950.053.11171746
ACGTCTG61350.050.6653815
CGTATGC48750.048.97244644
CACGTCT66800.046.635514
ACACGTC67900.046.03309613
CAGTCAC57150.045.09239627
GTATGCC53850.044.52636745
GTCTGAA72250.043.26049417
ATGCCGT56000.042.93903447
AGTCACC60000.042.08804728
CACACGT79450.039.34144612
CGTCCAT60500.039.06170735
GCCGTCT61350.038.85715549
TCCAGTC80000.038.3794725
CCAGTCA69450.038.34811426
CTCCAGT82500.037.3837224
CCGTCTT63750.037.28587750
TCTGAAC84650.036.92345418
Produced by FastQC (version 0.11.2)