FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12296753
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA398100.3237439997371664TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG59800.057.519545
CTCGTAT53350.057.2312944
TATGCCG62450.055.02168748
AGTCACA72100.050.52828
ACGTCTG86500.049.4546715
CGTATGC70800.049.16700446
CAGTCAC75800.048.47121427
GTATGCC71750.048.32269747
GTCACAG75300.047.41880829
ATGCCGT73150.046.9262449
CACAGTC76950.045.68470831
ACAGTCA76850.045.56458732
CACGTCT94600.045.32976514
GTCTGAA97150.044.246317
ACACGTC98250.043.82150713
CCAGTCA93400.041.55364626
CCGTCTT84100.040.77548652
TCCAGTC104100.040.69594625
GTCAACA86150.040.56656335
TCACAGT87250.040.41017530
Produced by FastQC (version 0.11.2)