FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10745216
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG923490.8594429372103828TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT114950.062.99939742
TCGTATG127050.062.83789443
TATGCCG130650.061.02578446
GTATGCC134550.059.30903245
CAGTCAC138850.059.2323527
CGTATGC136950.058.3455144
TGTAATC124950.057.7338536
ATGCCGT138750.057.4861947
TAATCTC125500.057.48163238
ACGTCTG154950.057.28577815
AGTCACC144650.056.285228
CTTGTAA142850.055.93311734
TTGTAAT144600.055.04172535
CCAGTCA153250.054.5445726
CACGTCT163900.054.39014414
CCGTCTT146500.054.0916350
GTAATCT134400.053.82920537
GTCTGAA165700.053.631517
GCCGTCT148700.053.36119549
TCTCGTA137550.052.54851541
Produced by FastQC (version 0.11.2)