FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11877390
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG562190.47332789442798456TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG87600.060.4153143
CTCGTAT83900.060.16003842
TATGCCG91050.058.5414346
CGTATGC94200.056.36504444
TATCTCG90000.056.3132439
ACGTCTG114500.056.04099315
AGTCACT101150.055.55888428
GTATGCC97450.054.8388145
ATGCCGT100200.053.160747
ACACGTC121750.052.931213
CACGTCT122550.052.44469514
CAGTCAC109550.052.1175527
CTTATCT98650.051.829737
GTCTGAA127700.050.54535317
TTATCTC101350.050.2106538
CCGTCTT108400.048.21616750
CACACGT133600.048.18549712
GCCGTCT108000.048.1072549
TCACTAG116950.047.5513830
CCAGTCA125200.047.3663526
Produced by FastQC (version 0.11.2)