FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10832088
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA274720.25361684653965144TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT38900.052.94951644
TCGTATG42800.052.1552245
TATGCCG45800.048.35848648
ACGTCTG59100.047.74907715
CGTATGC49350.045.37188346
GTCACAT52400.045.3616829
AGTCACA55450.043.8620328
GTATGCC52150.042.9993847
CAGTCAC57800.042.61588327
CACGTCT66350.042.58357614
ACACGTC68300.041.36779413
ATGCCGT56000.039.55033549
GTCTGAA73050.039.1029317
CCAGTCA69100.037.39425726
CACATGT64250.036.35102531
CACACGT78600.035.85920712
TCCAGTC79000.035.67764325
ATGTCAG66250.035.56673434
AGAATCT62250.034.1951939
TCTGAAC84500.033.80436718
Produced by FastQC (version 0.11.2)