FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4w-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4w-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10928615
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA304550.27867209156878525TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT40650.057.71212839
CTCGTAT43450.053.75533744
TCGTATG45600.053.641945
TATGCCG48300.051.49901648
TTCCGTA49800.049.5326436
TATCTCG47600.048.85214241
TCCGTAT50550.048.38868337
ACGTCTG65400.047.8972915
CCGTATC49500.047.463638
CGTATGC52650.046.78671646
CAGTCAC59150.044.38464727
AGTTCCG56700.043.99081834
AGTCACA59600.043.81799328
ACACGTC73450.042.97720313
ATGCCGT57800.042.795949
GTATGCC58200.042.67959647
CACGTCT74200.042.12414614
GTCACAG61700.041.37589629
GTTCCGT59850.041.2727735
GTCTGAA79650.039.63128717
Produced by FastQC (version 0.11.2)