FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12166959
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG548140.45051520268951345TruSeq Adapter, Index 3 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT134240.11033159559426477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT74900.059.74180642
TCGTATG80200.059.3642443
TATGCCG80150.058.9268246
ACTTAGG82250.058.8472132
AGTCACT91450.054.3606228
GTCACTT91300.054.1098529
GTATGCC87600.053.91556545
CGTATGC89800.052.59512744
ATGCCGT90700.052.3762447
TTAGGCA91850.052.35856634
CAGTCAC98250.051.511227
ACGTCTG112900.051.03055215
CACGTCT121200.047.56473514
GTCTGAA123850.046.79693217
GCCGTCT101600.046.5532149
ACACGTC125100.046.1370613
CCGTCTT104600.045.28399750
CCAGTCA117950.045.0137126
CTTAGGC107650.044.8981333
TCCAGTC129850.044.23605325
Produced by FastQC (version 0.11.2)