FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12768299
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG501470.39274612851719715TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT67700.058.3009542
TCGTATG76350.057.02783643
TATGCCG78900.054.65764246
CGTATGC79500.054.59379644
ACGTCTG109100.051.51107415
CAGTCAC91650.050.74051727
AGTCACA90600.050.52894228
GTATGCC86450.050.04519345
ATGCCGT90850.047.84628347
ACACGTC119350.047.26212313
CACGTCT119750.047.04552514
GTCACAT97300.046.19862729
GTCTGAA123400.045.62513717
TCACGAT98600.044.96078535
CCAGTCA111850.044.3218926
ATCACGA102650.043.92592234
CACGATC89350.043.7080336
TCCAGTC128350.043.56987425
CACACGT131500.042.9745312
CCGTCTT101450.042.64303250
Produced by FastQC (version 0.11.2)