FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12137219
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG674680.5558769269962089TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG111250.060.96866243
TATGCCG112600.060.7269246
CTCGTAT100650.060.36271742
CGTATGC118000.058.03584744
GTCACAC127450.057.19567529
GTATGCC123450.055.6693445
ATGCCGT125400.055.0226347
AGTCACA135350.054.34160228
TCACACT135250.053.769630
CACACTT134400.053.7246431
CAGTCAC141100.052.6405627
GCCGTCT131000.051.1430749
TTGAATC124500.050.57193836
ACGTCTG160400.050.522415
CCGTCTT134250.050.2390450
CTTGAAT145400.049.28197535
TCTCGTA125600.048.8123741
CCAGTCA156150.048.69373726
CACTTGA149500.048.3905733
TCCAGTC166650.048.316925
Produced by FastQC (version 0.11.2)