FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19664403
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG545980.27764890701233086TruSeq Adapter, Index 5 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT237960.12101053868759706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT79700.056.6216942
TCGTATG86200.056.23445543
TATGCCG90000.053.74346546
GTCACAC98000.051.29025729
CACAGTG101550.048.5460233
CGTATGC101950.047.64784644
ACACAGT104450.047.13211432
AGTCACA109150.047.030628
GTATGCC103850.046.776145
ACGTCTG138050.046.2813415
CACACAG108400.046.01933731
CAGTCAC114400.045.77697827
ATGCCGT107950.044.9336647
CACGTCT148650.043.12077714
ACACGTC154250.041.60044513
GTCTGAA157950.040.60328317
CCAGTCA143750.039.09440626
TCCAGTC163250.038.86242725
CTCCAGT166400.038.2096824
GCCGTCT127000.037.97638749
Produced by FastQC (version 0.11.2)