FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13000967
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG407640.3135459077774753TruSeq Adapter, Index 4 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT143750.11056869846681405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65750.055.56775343
CTCGTAT56100.055.2250542
TATGCCG66250.054.93900746
ACTGACC68500.053.13301532
AGTCACT73800.051.65453728
ACGTCTG100750.050.78108615
GTATGCC74900.048.4564445
CGTATGC75750.048.1406144
CACGTCT107050.047.88980514
CAGTCAC82450.047.6579827
ACACGTC108250.047.48640413
ATGCCGT77750.046.9010147
GTCTGAA111400.045.9261717
CTGACCA85450.042.59346433
CACACGT122150.042.02622612
TCCAGTC119350.041.97075325
CTCCAGT122100.041.25150324
CCAGTCA103050.041.01008226
TCTGAAC125250.040.90279418
CTGAACT125350.040.75991419
Produced by FastQC (version 0.11.2)