FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13312444
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG499570.3752654283465906TruSeq Adapter, Index 2 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT164340.12344840661864943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT70400.058.61326242
TATGCCG73500.057.8766446
TCGTATG74950.057.03463443
CGTATGC81100.052.79425444
TATCTCG78900.052.0360239
GTATGCC82650.051.92900545
CACCGAT86000.050.90568531
CAGTCAC91750.049.63305327
ACGTCTG105400.049.4572815
ATGCCGT86750.049.07406647
CGATGTA92450.046.7949134
CACGTCT113800.045.9282614
TGTATCT91150.045.45786337
GTCTGAA115550.045.23216617
AGTCACC100050.045.06730728
GTATCTC91400.044.95623438
ACACGTC116750.044.9158513
CCGATGT97650.044.37317733
GCCGTCT96400.043.73212449
TCCAGTC119250.043.39462725
Produced by FastQC (version 0.11.2)