FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12380538
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG335390.2709009899246705TruSeq Adapter, Index 7 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT136590.11032638484692668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125570.10142531770428716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT54050.054.5747342
TCGTATG56900.053.78145643
TATGCCG58850.052.05676346
CGTATGC61650.049.63690644
ACGTCTG89350.045.2520715
GTATGCC68200.045.07188445
CAGTCAC76450.043.77215227
ATGCCGT72750.042.63164547
CACGTCT98300.041.30827714
ACACGTC100050.040.58623513
GTCTGAA101100.040.40228717
TCCAGTC102650.039.25432625
GCCGTCT77700.039.2497349
CCAGTCA91150.038.5296226
CCGTCTT80400.037.9745550
TCTCGTA78400.037.75671441
AGTCACC86750.037.6603328
CTCCAGT109150.037.16954424
TCTGAAC111850.036.36496718
CATCTCG85700.035.30501639
Produced by FastQC (version 0.11.2)