FastQCFastQC Report
Fri 24 Feb 2017
HFJJCBGX2_n01_4s-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJJCBGX2_n01_4s-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12729544
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG865380.6798201098169738TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT114950.061.2293342
TCGTATG119150.060.4898843
TATGCCG119950.059.9398146
CGTATGC123000.058.455144
TATCTCG121800.057.84110339
GTCACGC128150.057.2597829
AGTCACG129350.057.02195428
GTATGCC128300.056.14747245
CGCCAAT129150.056.1219133
ATGCCGT131550.054.75795747
TCACGCC133650.054.64528730
CAGTCAC136950.054.6384427
GCCAATA134350.053.8739634
CACGCCA135650.053.68700831
ATATCTC134550.052.41072538
ACGTCTG159700.051.78042615
CCAATAT141150.051.22965635
GCCGTCT139350.051.17303549
TCTCGTA141500.050.0089341
CCAGTCA155950.049.26467526
Produced by FastQC (version 0.11.2)