FastQCFastQC Report
Fri 27 Sep 2019
HFJGYBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJGYBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244030008
Sequences flagged as poor quality0
Sequence length70
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA2346550.034.27263363
CCGTATC2390050.033.43630262
GCCGTAT2594550.029.13440161
CGCCGTA2603850.028.96347460
TCGCCGT3198450.025.54701859
GTATCAT3587350.022.34632164
GTCGCCG3865850.021.13621358
TGGTCGC4218750.018.7765156
GGTCGCC4274750.018.62174857
GTGGTCG4420050.018.22114855
TCGGTGG4562500.017.53126352
CTCGGTG4805700.017.03123351
CGGTGGT4718050.016.78812253
TCTCGGT5146850.016.48720650
AAGTACT2411650.016.4367164
AGTACTC2674050.016.0470735
GGTGGTC4984450.015.978654
CGGGGCA75700.015.76789217
TATCCTG747450.015.7151655
CGCGGAC4963500.015.59290232
Produced by FastQC (version 0.11.5)