FastQCFastQC Report
Fri 27 Sep 2019
HFJGYBGXC_n02_NLP7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJGYBGXC_n02_NLP7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117999
Sequences flagged as poor quality0
Sequence length70
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC200.009884804548.07
GCAGTTA200.009884804548.028
GGACGTA200.009884804548.06
TGTCCCC309.108206E-442.66666811
CATTGAG350.001943289336.571435
GGAGAGA400.003739985632.08
CCGTATC555.0335977E-429.0909162
CTTATGG555.0335977E-429.090913
GCCGTAT555.0335977E-429.0909161
GCCTACA608.371949E-426.6666661
GCGTTGA951.8633533E-523.57894713
CTTCAAG800.004460079620.01
TCGGTGG800.004460079620.052
TCTGCGT1501.4852703E-619.19999910
CTGCGTT1351.2903718E-518.96296311
TGCGTTG1401.7652354E-518.28571512
CCACTGC1251.541182E-417.9222
TCGCCGT900.00880459517.77777959
CGGTGGT900.00880459517.77777953
ACTGCTT1503.1941345E-517.06666624
Produced by FastQC (version 0.11.5)