FastQCFastQC Report
Fri 27 Sep 2019
HFJGYBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJGYBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244030008
Sequences flagged as poor quality0
Sequence length13
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGG15040480261.63373235639119No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTT952.3983193E-77.00219441
GTCTCGG952.3983193E-77.00219441
GAGCTGG706.316454E-57.0021941
GTCCTAC550.00182431857.0021941
GGTCCGC500.00562214677.0021941
GCCGGAG1209.349606E-107.0021941
GTACTGT852.220604E-67.0021941
GAGTTGC550.00182431857.0021941
GGTTGCC706.316454E-57.0021941
GTGGTAC550.00182431857.0021941
AGTACAC651.934191E-47.0021941
AGATCGG2950.06.8835131
AGTACGG1509.094947E-126.7687881
ATGCCGT7300.06.75981144
AGGCAGA1252.2628228E-96.7221061
GGGCGCT1206.859409E-96.7104361
AGTTGAC1051.9207255E-76.66875651
GGGCTCA951.7806578E-66.63365751
AGTCAGC905.4321226E-66.6131831
GCGCGAA905.4321226E-66.6131831
Produced by FastQC (version 0.11.5)